Site-Specific Recombinases: Methods and Protocols 1st ed. 2017 Edition

Ebook Details

Authors

Nikolai Eroshenko

Year 2017
Pages 342
Publisher Humana Press
Language en
ISBN 9781493971671
File Size 5.74 MB
File Format PDF
Download Counter 76
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Ebook Description

This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.